Antibacterial Potential of Plant-Extract Mediated Silver Nanoparticles Against Pathogenic Bacteria Isolated from Vaginosis
DOI:
https://doi.org/10.31530/cjnst.2026.2.2.2Keywords:
Bacterial Vaginosis, Green biosynthesized Nanoparticles, Purslane, AntibacterialsAbstract
Background: Bacterial Vaginosis (BV) is a prevalent bacterial dysbiosis worldwide. The emergence of multi-drug resistant (MDR) pathogens enhanced interest in nanomedicine as an alternative therapeutic approach.
Aims: This study investigated the incidence and etiology of BV, besides the antibacterial potential of Purslane (Portulaca oleracea L) combined silver nanoparticles (AgNPs) was evaluated.
Methodology: Eighty three women patients were screened for BV at public hospitals and private clinics. Bacterial identification and susceptibility were carried out using Vitek-2 system, molecular analysis and disk diffusion assay. Concurrently, Purslane-mediated AgNPs were synthesized by green biosynthesis, characterized by UV-Vis, XRD, FE-SEM, TEM and FTIR, and its antibacterial activity was examined by diffusion and microdilution assays.
Results: It was found that the incidence rate of BV is 57.8%, and the bacteriological analysis yielded 48 isolates across 11 bacterial species. Staphylococcus aureus was the most prevalent (20.83%), followed by Staphylococcus haemolyticus (18.75%), Escherichia coli (16.67%), Klebsiella pneumoniae (12.5%), Staphylococcus epidermidis (12.5%), Staphylococcus hominis (8.33%), and Klebsiella aerogenes, Vaginella massiliensis, Corynebacterium amycolatum, Enterococcus faecalis and Streptococcus agalactiae at (2.08%). Resistance against cefixime (68.75%), colistin (45.83) and amoxicillin (37.5) was observed across most of the isolates. On the other hand, characterizations confirmed the successful biosynthesis of quasi-spherical Purslane-mediated AgNPs at absorbance peak of 450 nm, with a diameter of 14.89 nm, FTIR spectroscopy revealed that the reduction of Ag⁺ to Ag⁰ and subsequent stabilization were driven by plant phenolics and proteins, characterized by significant intensity shifts in the hydroxyl (3406 cm⁻¹), amide (1631 cm⁻¹), and C–O (1012 cm⁻¹) vibrational bands. Notably, the Purslane-mediated AgNPs exhibited broad antibacterial efficacy against all BV bacterial species, with MIC ranging from 15.6 to 250 μg/ml.
Conclusion: this study reveals the development of MDR bacteria in BV and promising antibacterial activity of Purslane-mediated AgNPs.
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